mouse monoclonal antibodies against her3  (R&D Systems)

Journal: Scientific Reports

Article Title: Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers

doi: 10.1038/s41598-020-59514-1

Figure Lengend Snippet: Risk of worse breast cancer-specific or distant metastasis-free survival conferred by high protein levels HER3, EGFR, or HER3-EGFR protein expression in univariate Cox models stratified by hospital.

Article Snippet: For HER3, antigen retrieval was performed using the Dako Target Retrieval Solution at pH 9 in a 90 °C water bath for 35 min. Then, slides were incubated with monoclonal mouse anti-human HER3 antibody (DAK-H3-IC) at 1:25 for 30 min. For EGFR, antigen retrieval was performed using Proteinase K (Agilent, S3020) for 6 min on a hot rack.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers

doi: 10.1038/s41598-020-59514-1

Figure Lengend Snippet: Risk of worse breast cancer-specific or distant metastasis-free survival conferred by high protein levels of HER3, EGFR, or HER3-EGFR in age- and stage-adjusted Cox models stratified by hospital.

Article Snippet: For HER3, antigen retrieval was performed using the Dako Target Retrieval Solution at pH 9 in a 90 °C water bath for 35 min. Then, slides were incubated with monoclonal mouse anti-human HER3 antibody (DAK-H3-IC) at 1:25 for 30 min. For EGFR, antigen retrieval was performed using Proteinase K (Agilent, S3020) for 6 min on a hot rack.

Techniques:

Journal: Scientific Reports

Article Title: Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers

doi: 10.1038/s41598-020-59514-1

Figure Lengend Snippet: Predicted causal networks explaining observed gene expression differences based on combined HER3-EGFR protein expression.

Article Snippet: For HER3, antigen retrieval was performed using the Dako Target Retrieval Solution at pH 9 in a 90 °C water bath for 35 min. Then, slides were incubated with monoclonal mouse anti-human HER3 antibody (DAK-H3-IC) at 1:25 for 30 min. For EGFR, antigen retrieval was performed using Proteinase K (Agilent, S3020) for 6 min on a hot rack.

Techniques: Expressing, Activation Assay

Journal: World Journal of Oncology

Article Title: Synergistic Effects of Neratinib in Combination With Palbociclib or Miransertib in Brain Cancer Cells

doi: 10.14740/wjon1873

Figure Lengend Snippet: Surface Expression of Various Growth Factor Receptors in Human Brain Cancer Cell Line (Determined by Flow Cytometry)

Article Snippet: The mouse monoclonal antibodies against HER3 (MAB3481), HER4 (MAB11311), ALK7 (MAB77491) and HGF R/c-MET (MAB3582) were purchased from R&D Systems (Oxford, UK).

Techniques: Expressing, Cytometry, Control, Fluorescence

Journal: Cancer Cell International

Article Title: Establishment of primary human breast cancer cell lines using “pulsed hypoxia” method and development of metastatic tumor model in immunodeficient mice

doi: 10.1186/s12935-019-0766-5

Figure Lengend Snippet: Expression of HER2, HER3, Vimentin and Ki-67 in primary and cultured cells. a Analysis of surface expression of HER2 and HER3 by flow cytometry with specific antibodies. b Double immunofluorescence staining of Vimentin (green) and Ki-67 (red). Immunocytochemical detection (×40) in cancer and normal human cells

Article Snippet: FITC-conjugated mouse anti-human HER2 monoclonal (#2222020), FITC-conjugated mouse anti-human CD326 monoclonal (#2221020), PE -conjugated mouse anti-human CD324 monoclonal (#2220530), PE -conjugated mouse anti-human CD325 monoclonal (#2354025), allophycocyanin (APC)-conjugated mouse anti human HER3 monoclonal (#2223540), APC-conjugated mouse anti human CD146 monoclonal (#2310060) antibodies were purchased from Sony Biotechnology Inc. (San Jose, CA, USA).

Techniques: Expressing, Cell Culture, Flow Cytometry, Double Immunofluorescence Staining